Now showing items 21-40 of 56

    • Engineering metabolic pathways in plants by multigene transformation 

      Zorrilla López, Uxue; Masip Vilà, Gemma; Arjó Pont, Gemma; Bai, Chao; Banakar, Raviraj; Bassie Rene, Ludovic; Berman Quintana, Judit; Farré Martinez, Gemma; Miralpeix i Anglada, Bruna; Pérez Massot, Eduard; Sabalza Gallués, Maite; Sanahuja Solsona, Georgina; Vamvaka, Evangelia; Twyman, Richard M.; Christou, Paul; Zhu, Changfu; Capell Capell, Teresa (University of the Basque Country Press, 2013)
      Metabolic engineering in plants can be used to increase the abundance of specific valuable metabolites, but single-point interventions generally do not improve the yields of target metabolites unless that product is ...
    • Fast quantitative method for the analysis of carotenoids in transgenic maize 

      Rivera Vélez, Sol Maiam; Vilaró, Francisca; Zhu, Changfu; Bai, Chao; Farré Martinez, Gemma; Christou, Paul; Canela i Garayoa, Ramon (American Chemical Society, 2013)
      A fast method was developed to determine carotenoid content in transgenic maize seeds. The analysis was carried out using an ultrahigh-pressure liquid chromatograph coupled to a photodiode array detector and a mass ...
    • Freedom-to-operate analysis of a transgenic multivitamin corn variety 

      Zanga, Daniela; Capell Capell, Teresa; Zhu, Changfu; Christou, Paul; Thangaraj, Harry (John Wiley & Sons, 2016)
      In this article, we explore the intellectual property (IP) landscape relevant to the production and commercialization of Carolight™, a transgenic multivitamin corn variety created on humanitarian grounds to address ...
    • Functional characterization of the Gentiana lutea zeaxanthin epoxidase (GlZEP) promoter in transgenic tomato plants 

      Yang, Qingjie; Yuan, Dawei; Shi, Lianxuan; Capell Capell, Teresa; Bai, Chao; Wen, Nuan; Lu, Xiaodan; Sandmann, Gerhard; Christou, Paul; Zhu, Changfu (Springer Verlag, 2010)
      The accumulation of carotenoids in plants depends critically on the spatiotemporal expression profiles of the genes encoding enzymes in the carotenogenic pathway. We cloned and characterized the Gentiana lutea zeaxanthin ...
    • Genome editing in cereal crops: an overview 

      Matres, Jerlie Mhay; Hilscher, Julia; Datta, Akash; Armario-Nájera, Victoria; Baysal, Can; He, Wenshu; Huang, Xin; Zhu, Changfu; Valizadeh, R.; Trijatmiko, Kurniawan R.; Capell Capell, Teresa; Christou, Paul; Stoger, Eva; Slamet-Loedin, Inez H. (Springer, 2021)
      Genome-editing technologies offer unprecedented opportunities for crop improvement with superior precision and speed. This review presents an analysis of the current state of genome editing in the major cereal crops- rice, ...
    • High-carotenoid maize: development of plant biotechnology prototypes for human and animal health and nutrition 

      Zhu, Changfu; Farré Martinez, Gemma; Zanga, Daniela; Lloveras Vilamanyà, Jaume; Michelena Bárcena, Antonio; Ferrio Díaz, Juan Pedro; Voltas Velasco, Jordi; Slafer, Gustavo A.; Savin, Roxana; Albajes Garcia, Ramon; Eizaguirre Altuna, Matilde; López Alonso, Carmen; Cantero-Martínez, Carlos; Díaz Gómez, Joana; Nogareda, Carmina; Moreno Martínez, José Antonio; Angulo Asensio, Eduardo; Estany Illa, Joan; Pena i Subirà, Ramona Natacha; Tor i Naudí, Marc; Portero Otín, Manuel; Eritja Sánchez, Núria; Arjó Pont, Gemma; Serrano Casasola, José Carlos Enrique; Matias-Guiu, Xavier; Twyman, Richard M.; Sandmann, Gerhard; Capell Capell, Teresa; Christou, Paul (Springer Verlag, 2017-06-01)
      Carolight® is a transgenic maize variety that accumulates extraordinary levels of carotenoids, including those with vitamin A activity. The development of Carolight® maize involved the technical implementation of a novel ...
    • High-value products from transgenic maize 

      Naqvi, Shaista; Ramessar, Koreen; Farré Martinez, Gemma; Sabalza Gallués, Maite; Miralpeix i Anglada, Bruna; Twyman, Richard M.; Capell Capell, Teresa; Zhu, Changfu; Christou, Paul (Elsevier, 2011)
      Maize (also known as corn) is a domesticated cereal grain that has been grown as food and animal feed for tens of thousands of years. It is currently the most widely grown crop in the world, and is used not only for food/feed ...
    • Identification of line-specific strategies for improving carotenoid production in synthetic maize through data-driven mathematical modeling 

      Comas, Jorge; Benfeitas, Rui; Vilaprinyo Terré, Ester; Sorribas Tello, Albert; Solsona Tehàs, Francesc; Farré Martinez, Gemma; Berman Quintana, Judit; Zorrilla López, Uxue; Capell Capell, Teresa; Sandmann, Gerhard; Zhu, Changfu; Christou, Paul; Alves, Rui (Wiley online library, 2016)
      Plant synthetic biology is still in its infancy. However, synthetic biology approaches have been used to manipulate and improve the nutritional and health value of staple food crops such as rice, potato and maize. With ...
    • Inactivation of rice starch branching enzyme IIb triggers broad and unexpected changes in metabolism by transcriptional reprogramming 

      Baysal, Can; He, Wenshu; Drapal, Margit; Villorbina Noguera, Gemma; Medina Piles, Vicente; Capell Capell, Teresa; Khush, Gurdev S.; Zhu, Changfu; Fraser, Paul; Christou, Paul (National Academy of Sciences, 2020-10-05)
      Starch properties can be modified by mutating genes responsible for the synthesis of amylose and amylopectin in the endosperm. However, little is known about the effects of such targeted modifications on the overall starch ...
    • Influence of cooking conditions on carotenoid content and stability in porridges prepared from high-carotenoid maize 

      Díaz Gómez, Joana; Ramos Girona, Antonio J.; Zhu, Changfu; Martín Belloso, Olga; Soliva-Fortuny, Robert (Springer, 2017-03-29)
      Maize is a staple food crop in many developing countries, hence becoming an attractive target for biofortification programs toward populations at risk of micronutrient deficiencies. A South African white endosperm maize ...
    • Metabolic engineering of ketocarotenoid biosynthesis in higher plants 

      Zhu, Changfu; Naqvi, Shaista; Capell Capell, Teresa; Christou, Paul (Elsevier, 2009)
      Ketocarotenoids such as astaxanthin and canthaxanthin have important applications in the nutraceutical, cosmetic, food and feed industries. Astaxanthin is derived from b-carotene by 3-hydroxylation and 4-ketolation at ...
    • Mice fed on a diet enriched with genetically engineered multivitamin corn show no sub-acute toxic effects and no sub-chronic toxicity 

      Arjó Pont, Gemma; Capell Capell, Teresa; Matias-Guiu, Xavier; Zhu, Changfu; Christou, Paul; Piñol Felis, Carme (Wiley-BlackwellJohn Wiley & Sons, 2012-12)
      Multivitamin corn is a novel genetically engineered variety that simultaneously produces high levels of beta-carotene, ascorbate and folate, and therefore has the potential to address simultaneously multiple micronutrient ...
    • Molecular characterization of the Arginine decarboxylase gene family in rice 

      Peremartí, Ariadna; Bassie Rene, Ludovic; Zhu, Changfu; Christou, Paul; Capell Capell, Teresa (Springer Verlag, 2010)
      Arginine decarboxylase (ADC) is a key enzyme in plants that converts arginine into putrescine, an important mediator of abiotic stress tolerance. Adc genes have been isolated from a number of dicotyledonous plants but ...
    • Multilevel interactions between native and ectopic isoprenoid pathways affect global metabolism in rice 

      Pérez, Lucía; Alves, Rui; Perez‑Fons, Laura; Albacete, Alfonso; Farré Martinez, Gemma; Soto, Erika; Vilaprinyó, Ester; Martínez‑Andújar, Cristina; Basallo, Oriol; Fraser, Paul D.; Medina, Vicente; Zhu, Changfu; Capell Capell, Teresa; Christou, Paul (Springer, 2022)
      MVA pathway could be circumvented by express- ing an ectopic plastidial MVA pathway that increases the accumulation of IPP and DMAPP in plastids. We therefore introduced genes encoding the plastid- targeted enzymes ...
    • Nutritionally enhanced crops and food security: scientific achievements versus political expediency 

      Farré Martinez, Gemma; Twyman, Richard M.; Zhu, Changfu; Capell Capell, Teresa; Christou, Paul (Elsevier, 2011)
      Genetic engineering (GE) is one of a raft of strategies that can be used to tackle malnutrition. Recent scientific advances have shown that multiple deficiencies can be tackled simultaneously using engineered plant varieties ...
    • Nutritious crops producing multiple carotenoids – a metabolic balancing act 

      Farré Martinez, Gemma; Bai, Chao; Twyman, Richard M.; Capell Capell, Teresa; Christou, Paul; Zhu, Changfu (Elsevier, 2011)
      Plants and microbes produce multiple carotenoid pigments with important nutritional roles in animals. By unraveling the basis of carotenoid biosynthesis it has become possible to modulate the key metabolic steps in plants ...
    • Patterns of CRISPR/Cas9 activity in plants, animals and microbes 

      Bortesi, Luisa; Zhu, Changfu; Zischewski, Julia; Pérez Álvarez, Lucía; Bassie Rene, Ludovic; Nadi, Riad; Forni, Giobbe; Lade Lee, Sarah Boyd; Soto, Erika; Jin, Xin; Medina Piles, Vicente; Villorbina Noguera, Gemma; Muñoz Gràcia, Pilar; Farré Martinez, Gemma; Fischer, Rainer; Twyman, Richard M.; Capell Capell, Teresa; Christou, Paul; Schillberg, Stefan (Wiley, 2016)
      The CRISPR/Cas9 system and related RNA-guided endonucleases can introduce double-strand breaks (DSBs) at specific sites in the genome, allowing the generation of targeted mutations in one or more genes as well as more ...
    • Promoter diversity in multigene transformation 

      Peremartí, Ariadna; Twyman, Richard M.; Gómez Galera, Sonia; Naqvi, Shaista; Farré Martinez, Gemma; Sabalza Gallués, Maite; Miralpeix i Anglada, Bruna; Dashevskaya, Svetlana; Yuan, Dawei; Ramessar, Koreen; Christou, Paul; Zhu, Changfu; Bassie Rene, Ludovic; Capell Capell, Teresa (Springer Verlag, 2010)
      Multigene transformation (MGT) is becoming routine in plant biotechnology as researchers seek to generate more complex and ambitious phenotypes in transgenic plants. Every nuclear transgene requires its own promoter, ...
    • Provitamin A carotenoids from an engineered high-carotenoid maize are bioavailable and zeaxanthin does not compromise β-carotene absorption in poultry 

      Díaz Gómez, Joana; Moreno Martínez, José Antonio; Angulo Asensio, Eduardo; Sandmann, Gerhard; Zhu, Changfu; Capell Capell, Teresa; Nogareda, Carmina (Springer International Publishing, 2017)
      High-carotenoid (HC) maize, a biofortified staple crop which accumulates β-carotene, β-cryptoxanthin, lutein and zeaxanthin, was used as a feed component in a chicken feeding trial to assess the bioavailability of provitamin ...
    • Recognition motifs rather than phylogenetic origin influence the ability of targeting peptides to import nuclear-encoded recombinant proteins into rice mitochondria 

      Baysal, Can; Pérez-González, Ana; Eseverri, Álvaro; Jiang, Xi; Medina Piles, Vicente; Caro, Elena; Rubio, Luis; Christou, Paul; Zhu, Changfu (Springer Nature, 2019-10-10)
      Mitochondria fulfil essential functions in respiration and metabolism as well as regulating stress responses and apoptosis. Most native mitochondrial proteins are encoded by nuclear genes and are imported into mitochondria ...