Now showing items 1-17 of 17

    • A peroxisomal glutathione transferase of Saccharomyces cerevisiae is functionally related to sulfur amino acid metabolism 

      Barreto Parra, Lina Patricia; Garcera, Ana; Jansson, Kristina; Sunnerhagen, Per; Herrero Perpiñán, Enrique (American Society for Microbiology, 2006)
      Saccharomyces cerevisiae cells contain three omega-class glutathione transferases with glutaredoxin activity (Gto1, Gto2, and Gto3), in addition to two glutathione transferases (Gtt1 and Gtt2) not classifiable into standard ...
    • An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast 

      Bellí i Martínez, Gemma; Garí Marsol, Eloi; Piedrafita Llorens, Lídia; Aldea, Martí; Herrero Perpiñán, Enrique (Oxford University Press, 1998)
      We have developed an activator/repressor expression system for budding yeast in which tetracyclines control in opposite ways the ability of tetR-based activator and repressor molecules to bind tetO promoters. This combination ...
    • Bck2 is a phase-independent activator of cell cycle-regulated genes in yeast 

      Ferrezuelo, Francisco; Aldea, Martí; Futcher, Bruce (Landes Bioscience, 2009)
      During the cell division cycle of the yeast Saccharomyces cerevisiae, the G1-to-S transition depends upon the activation of two transcription factors (SBF and MBF), which are responsible for the cell cycle-regulated ...
    • Comparative genomics of yeast species: new insights into their biology 

      Herrero Perpiñán, Enrique; Torre Ruiz, M. A. de la; Valentín, Eulogio (Sociedad Española de Microbiología, 2003)
      The genomes of two hemiascomycetous yeasts (Saccharomyces cerevisiae and Candida albicans) and one archiascomycete (Schizosaccharomyces pombe) have been completely sequenced and the genes have been annotated. In addition, ...
    • Evolutionary relationships between Saccharomyces cerevisiae and other fungal species as determined from genome comparisons 

      Herrero Perpiñán, Enrique (Elsevier España, 2005)
      The increasing number of fungal genomes whose sequence has been completed permits their comparison both at the nucleotide and protein levels. The informa- tion thus obtained improves our knowledge on evolutionary ...
    • Glutaredoxins in fungi 

      Herrero Perpiñán, Enrique; Ros Salvador, Joaquim; Tamarit Sumalla, Jordi; Bellí i Martínez, Gemma (Springer, 2006)
      Glutaredoxins (GRXs) can be subdivided into two subfamilies: dithiol GRXs with the CPY/FC active site motif, and monothiol GRXs with the CGFS motif. Both subfamilies share a thioredoxin-fold structure. Monothiol GRXs exist ...
    • Grx5 glutaredoxin plays a central role in protection against protein oxidative damage in Saccharomyces cerevisiae 

      Rodríguez Manzanaque, Maria Teresa; Ros Salvador, Joaquim; Cabiscol Català, Elisa; Sorribas Tello, Albert; Herrero Perpiñán, Enrique (American Society for Microbiology, 1999)
      Glutaredoxins are members of a superfamily of thiol disulfide oxidoreductases involved in maintaining the redox state of target proteins. In Saccharomyces cerevisiae, two glutaredoxins (Grx1 and Grx2) containing a cysteine ...
    • Isolation and characterization of Saccharomyces cerevisiae mutants resistant to aculeacin A 

      Font de Mora, Jaime; Gil, Rosario; Sentandreu, Rafael; Herrero Perpiñán, Enrique (American Society for Microbiology, 1991)
      Aculeacin A is a lipopeptide that inhibits ,B-glucan synthesis in yeasts. A number of Saccharomyces cerevisiae mutants resistant to this antibiotic were isolated, and four loci (ACRI, ACR2, ACR3, and ACR4) whose products ...
    • Monothiol glutaredoxins: a common domain for multiple functions 

      Herrero Perpiñán, Enrique; Torre Ruiz, M. A. de la (Springer, 2007)
      Monothiol glutaredoxins with the CGFS sequence at the active site are widespread among prokaryotes and eukaryotes. Two subclasses exist, those with a single glutaredoxin domain and those with a thioredoxin-like region ...
    • Saccharomyces cerevisiae as a model organism: a comparative study 

      Karathia, Hiren; Vilaprinyo Terré, Ester; Sorribas Tello, Albert; Alves, Rui (Public Library of Science (PLoS), 2011)
      Background: Model organisms are used for research because they provide a framework on which to develop and optimize methods that facilitate and standardize analysis. Such organisms should be representative of the living ...
    • Saccharomyces cerevisiae Grx6 and Grx7 are monothiol glutaredoxins associated with the early secretory pathway 

      Izquierdo Álvarez, Alicia; Casas Herranz, Celia; Mühlenhoff, Ulrich; Lillig, Christopher Horst; Herrero Perpiñán, Enrique (American Society for Microbiology, 2008)
      Saccharomyces cerevisiae Grx6 and Grx7 are two monothiol glutaredoxins whose active-site sequences (CSYS and CPYS, respectively) are reminiscent of the CPYC active-site sequence of classical dithiol glutaredoxins. Both ...
    • Selenite-induced cell death in Saccharomyces cerevisiae: protective role of glutaredoxins 

      Izquierdo Álvarez, Alicia; Casas Herranz, Celia; Herrero Perpiñán, Enrique (Society for General Microbiology, 2010)
      Unlike in higher organisms, selenium is not essential for growth in Saccharomyces cerevisiae. In this species, it causes toxic effects at high concentrations. In the present study, we show that when supplied as selenite ...
    • Structural and functional diversity of glutaredoxins in yeast 

      Herrero Perpiñán, Enrique; Bellí i Martínez, Gemma; Casas Herranz, Celia (Bentham Science Publishers, 2010)
      Glutaredoxins are defined as thiol disulfide oxidoreductases that reduce disulfide bonds employing reduced glutathione as electron donor. They constitute a complex family of proteins with a diversity of enzymatic and ...
    • The AMPK family member Snf1 protects Saccharomyces cerevisiae cells upon glutatione oxidation 

      Pérez Sampietro, María; Casas Herranz, Celia; Herrero Perpiñán, Enrique (Public Library of Science, 2013)
      The AMPK/Snf1 kinase has a central role in carbon metabolism homeostasis in Saccharomyces cerevisiae. In this study, we show that Snf1 activity, which requires phosphorylation of the Thr210 residue, is needed for protection ...
    • The cell cycle–regulated genes of schizosaccharomyces pombe 

      Oliva, Anna; Rosebrock, Adam; Ferrezuelo, Francisco; Pyne, Saumyadipta; Chen, Haiying; kiena, Steve; Futcher, Bruce; Leatherwood, Janet (Public Library of Science (PLoS), 2005)
      Many genes are regulated as an innate part of the eukaryotic cell cycle, and a complex transcriptional network helps enable the cyclic behavior of dividing cells. This transcriptional network has been studied in Saccharomyces ...
    • Tor1, Sch9 and PKA downregulation in quiescence rely on Mtl1 to preserve mitochondrial integrity and cell survival 

      Sundaram, Venkatraghavan; Ivanova Petkova, Mima; Pujol Carrión, Núria; Boada Pallàs, Jordi; Torre Ruiz, M. A. de la (John Wiley & Sons, 2015-07-22)
      Here we show that Mtl1, member of the cell wall integrity pathway of Saccharomyces cerevisiae, plays a positive role in chronological life span (CLS). The absence of Mtl1 shortens CLS and causes impairment in the mitochondrial ...
    • Whi3 regulates morphogenesis in budding yeast by enhancing Cdk functions in apical growth 

      Colomina i Gabarrella, Neus; Ferrezuelo, Francisco; Vergés, Emili; Aldea, Martí; Garí Marsol, Eloi (Landes Bioscience, 2009)
      The Whi3 protein is associated with the endoplasmic reticulum, interacts with Cdc28, the budding-yeast Cdk, binds the mRNA of cyclin CLN3 and prevents accumulation of the Cdc28-Cln3 in the nucleus until late G1. Besides ...