Now showing items 1-15 of 15

    • A peroxisomal glutathione transferase of Saccharomyces cerevisiae is functionally related to sulfur amino acid metabolism 

      Barreto Parra, Lina Patricia; Garcera, Ana; Jansson, Kristina; Sunnerhagen, Per; Herrero Perpiñán, Enrique (American Society for Microbiology, 2006)
      Saccharomyces cerevisiae cells contain three omega-class glutathione transferases with glutaredoxin activity (Gto1, Gto2, and Gto3), in addition to two glutathione transferases (Gtt1 and Gtt2) not classifiable into standard ...
    • An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast 

      Bellí i Martínez, Gemma; Garí Marsol, Eloi; Piedrafita Llorens, Lídia; Aldea, Martí; Herrero Perpiñán, Enrique (Oxford University Press, 1998)
      We have developed an activator/repressor expression system for budding yeast in which tetracyclines control in opposite ways the ability of tetR-based activator and repressor molecules to bind tetO promoters. This combination ...
    • Comparative genomics of yeast species: new insights into their biology 

      Herrero Perpiñán, Enrique; Torre Ruiz, M. A. de la; Valentín, Eulogio (Sociedad Española de Microbiología, 2003)
      The genomes of two hemiascomycetous yeasts (Saccharomyces cerevisiae and Candida albicans) and one archiascomycete (Schizosaccharomyces pombe) have been completely sequenced and the genes have been annotated. In addition, ...
    • Glutaredoxins in fungi 

      Herrero Perpiñán, Enrique; Ros Salvador, Joaquim; Tamarit Sumalla, Jordi; Bellí i Martínez, Gemma (Springer, 2006)
      Glutaredoxins (GRXs) can be subdivided into two subfamilies: dithiol GRXs with the CPY/FC active site motif, and monothiol GRXs with the CGFS motif. Both subfamilies share a thioredoxin-fold structure. Monothiol GRXs exist ...
    • Grx5 is a mitochondrial glutaredoxin required for the activity of iron/sulfur enzymes 

      Rodríguez-Manzaneque Martínez, María Teresa; Tamarit Sumalla, Jordi; Bellí i Martínez, Gemma; Ros Salvador, Joaquim; Herrero Perpiñán, Enrique (American Society for Cell Biology, 2002)
      Yeast cells contain a family of three monothiol glutaredoxins: Grx3, 4, and 5. Absence of Grx5 leads to constitutive oxidative damage, exacerbating that caused by external oxidants. Phenotypic defects associated with the ...
    • Heat shock response in yeast involves changes in both transcription rates and mRNA stabilities 

      Castells i Roca, Laia; García-Martínez, José; Moreno, Joaquín; Herrero Perpiñán, Enrique; Bellí i Martínez, Gemma; Pérez-Ortín, José E. (Public Library of Science (PLoS), 2011)
      We have analyzed the heat stress response in the yeast Saccharomyces cerevisiae by determining mRNA levels and transcription rates for the whole transcriptome after a shift from 25uC to 37uC. Using an established mathematical ...
    • Isolation and characterization of Saccharomyces cerevisiae mutants resistant to aculeacin A 

      Font de Mora, Jaime; Gil, Rosario; Sentandreu, Rafael; Herrero Perpiñán, Enrique (American Society for Microbiology, 1991)
      Aculeacin A is a lipopeptide that inhibits ,B-glucan synthesis in yeasts. A number of Saccharomyces cerevisiae mutants resistant to this antibiotic were isolated, and four loci (ACRI, ACR2, ACR3, and ACR4) whose products ...
    • Monothiol glutaredoxins: a common domain for multiple functions 

      Herrero Perpiñán, Enrique; Torre Ruiz, M. A. de la (Springer, 2007)
      Monothiol glutaredoxins with the CGFS sequence at the active site are widespread among prokaryotes and eukaryotes. Two subclasses exist, those with a single glutaredoxin domain and those with a thioredoxin-like region ...
    • ORMDL proteins are a conserved new family of endoplasmic reticulum membrane proteins 

      Hjelmqvist, Lars; Tuson, Miquel; Marfany i Nadal, Gemma; Herrero Perpiñán, Enrique; Balcells Comas, Susana; González, Roser (BioMed Central, 2002)
      Background: Annotations of completely sequenced genomes reveal that nearly half of the genes identified are of unknown function, and that some belong to uncharacterized gene families. To help resolve such issues, information ...
    • Saccharomyces cerevisiae Grx6 and Grx7 are monothiol glutaredoxins associated with the early secretory pathway 

      Izquierdo Álvarez, Alicia; Casas Herranz, Celia; Mühlenhoff, Ulrich; Lillig, Christopher Horst; Herrero Perpiñán, Enrique (American Society for Microbiology, 2008)
      Saccharomyces cerevisiae Grx6 and Grx7 are two monothiol glutaredoxins whose active-site sequences (CSYS and CPYS, respectively) are reminiscent of the CPYC active-site sequence of classical dithiol glutaredoxins. Both ...
    • Selenite-induced cell death in Saccharomyces cerevisiae: protective role of glutaredoxins 

      Izquierdo Álvarez, Alicia; Casas Herranz, Celia; Herrero Perpiñán, Enrique (Society for General Microbiology, 2010)
      Unlike in higher organisms, selenium is not essential for growth in Saccharomyces cerevisiae. In this species, it causes toxic effects at high concentrations. In the present study, we show that when supplied as selenite ...
    • Structural and functional diversity of glutaredoxins in yeast 

      Herrero Perpiñán, Enrique; Bellí i Martínez, Gemma; Casas Herranz, Celia (Bentham Science Publishers, 2010)
      Glutaredoxins are defined as thiol disulfide oxidoreductases that reduce disulfide bonds employing reduced glutathione as electron donor. They constitute a complex family of proteins with a diversity of enzymatic and ...
    • The AMPK family member Snf1 protects Saccharomyces cerevisiae cells upon glutatione oxidation 

      Pérez Sampietro, María; Casas Herranz, Celia; Herrero Perpiñán, Enrique (Public Library of Science, 2013)
      The AMPK/Snf1 kinase has a central role in carbon metabolism homeostasis in Saccharomyces cerevisiae. In this study, we show that Snf1 activity, which requires phosphorylation of the Thr210 residue, is needed for protection ...
    • The transcriptional network activated by Cln3 cyclin at the G1-to-S transition of the yeast cell cycle 

      Ferrezuelo, Francisco; Colomina i Gabarrella, Neus; Futcher, Bruce; Aldea, Martí (BioMed Central, 2010)
      Background: The G1-to-S transition of the cell cycle in the yeast Saccharomyces cerevisiae involves an extensive transcriptional program driven by transcription factors SBF (Swi4-Swi6) and MBF (Mbp1-Swi6). Activation of ...
    • Zim17/Tim15 links mitochondrial iron–sulfur cluster biosynthesis to nuclear genome stability 

      Díaz de la Loza, María del Carmen; Gallardo, Mercedes; García-Rubio, María Luisa; Izquierdo, Alicia; Herrero Perpiñán, Enrique; Aguilera, Andrés; Wellinger, Ralf Erik (Oxford University Press, 2011)
      Genomic instability is related to a wide-range of human diseases. Here, we show that mitochondrial iron–sulfur cluster biosynthesis is important for the maintenance of nuclear genome stability in Saccharomyces cerevisiae. ...