Now showing items 1-17 of 17

    • A SUMO-Dependent Step during Establishment of Sister Chromatid Chohesion 

      Almedawar, Seba; Colomina i Gabarrella, Neus; Bermúdez López, Marcelino; Pociño Merino, Irene; Torres Rosell, Jordi (Elsevier, 2012-09-11)
      Cohesin is a protein complex that ties sister DNA molecules from the time of DNA replication until the metaphase to anaphase transition. Current models propose that the association of the Smc1, Smc3, and Scc1/Mcd1 subunits ...
    • ATPase-dependent control of the Mms21 SUMO ligase during DNA repair 

      Bermúdez López, Marcelino; Pociño Merino, Irene; Sánchez, Humberto; Bueno, Andrés; Guasch, Clàudia; Almedawar, Seba; Brú i Virgili, Sergi; Garí Marsol, Eloi; Wyman, Claire; Reverter Cendrós, David; Colomina i Gabarrella, Neus; Torres Rosell, Jordi (Plos Biology, 2015-03-12)
      Modification of proteins by SUMO is essential for the maintenance of genome integrity. During DNA replication, the Mms21-branch of the SUMO pathway counteracts recombination intermediates at damaged replication forks, thus ...
    • Can eukaryotic cells monitor the presence of unreplicated DNA? 

      Torres Rosell, Jordi; Piccoli, Giacomo De; Aragón, Luis (BioMed Central, 2007)
      Completion of DNA replication before mitosis is essential for genome stability and cell viability. Cellular controls called checkpoints act as surveillance mechanisms capable of detecting errors and blocking cell cycle ...
    • Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin 

      Pérez Fusté, Noel; Fernández Hernández, Rita; Cemeli, Tània; Mirantes Barbeito, Cristina; Pedraza González, Neus; Rafel i Borrell, Marta; Torres Rosell, Jordi; Colomina i Gabarrella, Neus; Ferrezuelo, Francisco; Dolcet Roca, Xavier; Garí Marsol, Eloi (Nature Research, 2016-05-16)
      Cyclin D1 (Ccnd1) together with its binding partner Cdk4 act as a transcriptional regulator to control cell proliferation and migration, and abnormal Ccnd1 . Cdk4 expression promotes tumour growth and metastasis. While ...
    • DNA activates the Nse2/Mms21 SUMO E3 ligase in the Smc5/6 complex 

      Varejao, Nathalia; Ibars Estiarte, Eva Irene; Lascorz, Jara; Colomina i Gabarrella, Neus; Torres Rosell, Jordi; Reverter Cendrós, David (EMBO Press, 2018-06-15)
      Modification of chromosomal proteins by conjugation to SUMO is a key step to cope with DNA damage and to maintain the integrity of the genome. The recruitment of SUMO E3 ligases to chromatin may represent one layer of ...
    • Pkc1 and the upstream elements of the cell integrity pathway in Saccharomyces cerevisiae, Rom2 and Mtl1, are required for cellular responses to oxidative stress 

      Vilella Mitjana, Felipe; Herrero Perpiñán, Enrique; Torres Rosell, Jordi; Torre Ruiz, M. A. de la (The American Society for Biochemistry and Molecular Biology, 2005)
      In this study we analyze the participation of the PKC1-MAPK cell integrity pathway in cellular re- sponses to oxidative stress in Saccharomyces cerevisiae. Evidence is presented demonstrating that only Pkc1 and the ...
    • Purified Smc5/6 Complex Exhibits DNA Substrate Recognition and Compaction 

      Gutierrez Escribano, Pilar; Hormeño, Silvia; Madariaga Marcos, Julene; Solé Soler, Roger; O'Reilly, Francis J.; Morris, Kyle; Aicart Ramos, Clara; Aramayo, Ricardo; Montoya, Alex; Kramer, Holger; Rappsilber, Juri; Torres Rosell, Jordi; Moreno Herrero, Fernando; Aragon, Luis (Elsevier, 2020-12-17)
      Eukaryotic SMC complexes, cohesin, condensin, and Smc5/6, use ATP hydrolysis to power a plethora of functions requiring organization and restructuring of eukaryotic chromosomes in interphase and during mitosis. The Smc5/6 ...
    • Regulation of the cell integrity pathway by rapamycin-sensitive TOR function in budding yeast 

      Torres Rosell, Jordi; Di Como, Charles J.; Herrero Perpiñán, Enrique; Torre Ruiz, M. A. de la (The American Society for Biochemistry and Molecular Biology, 2002)
      The TOR (target of rapamycin) pathway controls cell growth in response to nutrient availability in eukary- otic cells. Inactivation of TOR function by rapamycin or nutrient exhaustion is accompanied by triggering various ...
    • Sgs1’s roles in DNA end resection, HJ dissolution, and crossover suppression require a two-step SUMO regulation dependent on Smc5/6 

      Bermúdez López, Marcelino; Villoria, María Teresa; Esteras, Miguel; Jarmuz, Adam; Torres Rosell, Jordi; Clemente Blanco, Andrés; Aragón, Luis (Cold Spring Harbor Laboratory Press, 2016)
      The RecQ helicase Sgs1 plays critical roles during DNA repair by homologous recombination, fromend resection to Holliday junction (HJ) dissolution. Sgs1 has both pro- and anti-recombinogenic roles, and therefore its ...
    • Sit4 is required for proper modulation of the biological functions mediated by Pkc1 and the cell integrity pathway in Saccharomyces cerevisiae 

      Torre Ruiz, M. A. de la; Torres Rosell, Jordi; Ariño Carmona, Joaquín; Herrero Perpiñán, Enrique (The American Society for Biochemistry and Molecular Biology, 2002)
      Maintenance of cellular integrity in Saccharomyces cerevisiae is carried out by the activation of the protein kinase C-mediated mitogen-activated protein kinase (PKC1-MAPK) pathway. Here we report that correct down-regulation ...
    • Smc5/6, an atypical SMC complex with two RING-type subunits 

      Solé-Soler, Roger; Torres Rosell, Jordi (Portland Press, 2020-10-30)
      Smc5/6, an atypical SMC complex with two RING-type subunits Roger Solé-Soler ; Jordi Torres-Rosell Crossmark: Check for Updates Biochem Soc Trans (2020) 48 (5): 2159-2171. https://doi.org/10.1042/BST20200389 Article history ...
    • Smc5–Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination 

      De Piccoli, Giacomo; Cortes Ledesma, Felipe; Ira, Gregory; Torres Rosell, Jordi; Uhle, Stefan; Farmer, Sarah; Hwang, Ji-Young; Machín, Félix; Ceschia, Audrey; McAleenan, Alexandra; Cordon Preciado, Violeta; Clemente Blanco, Andrés; Vilella Mitjana, Felipe; Ullal, Pranav; Jarmuz, Adam; Leitao, Beatriz; Bressan, Debra; Dotiwala, Farokh; Papusha, Alma; Zhao, Xiaolan; Myung, Kyungjae; Haber, James E.; Aguilera, Andrés; Aragón, Luis (Nature, 2006)
      DNA double-strand breaks (DSB) can arise during DNA replication, or after exposure to DNA-damaging agents, and their correct repair is fundamental for cell survival and genomic stability. Here, we show that the ...
    • Spindle-independent condensation-mediated segregation of yeast ribosomal DNA in late anaphase 

      Machín, Félix; Torres Rosell, Jordi; Jarmuz, Adam; Aragón, Luis (The Rockefeller University Press, 2005)
      Mitotic cell division involves the equal segregation of all chromosomes during anaphase. The presence of ribosomal DNA (rDNA) repeats on the right arm of chromosome XII makes it the longest in the budding yeast genome. ...
    • Sumoylation of Smc5 Promotes Error-free Bypass at Damaged Replication Forks 

      Zapatka, Mariel; Pociño Merino, Irene; Heluani-Gahete, Hayat; Bermúdez López, Marcelino; Tarrés, Marc; Ibars Estiarte, Eva Irene; Solé-Soler, Roger; Gutiérrez-Escribano, Pilar; Apostolova, Sonia; Casas Herranz, Celia; Aragon, Luis; Wellinger, Ralf Erik; Colomina i Gabarrella, Neus; Torres Rosell, Jordi (Elsevier, 2019)
      Replication of a damaged DNA template can threaten the integrity of the genome, requiring the use of various mechanisms to tolerate DNA lesions. The Smc5/6 complex, together with the Nse2/Mms21 SUMO ligase, plays essential ...
    • The Aurora-B-dependent NoCut checkpoint prevents damage of anaphase bridges after DNA replication stress 

      Amaral, Nuno; Vendrell, Alexandre; Maier, Michael; Kumar, Arun; Mendoza, Manuel; Funaya, Charlotta; Geli, María Isabel; Idrissi, Fatima-Zahra; Colomina i Gabarrella, Neus; Torres Rosell, Jordi (Nature, 2016)
      Anaphase chromatin bridges can lead to chromosome breakage if not properly resolved before completion of cytokinesis. The NoCut checkpoint, which depends on Aurora B at the spindle midzone, delays abscission in response ...
    • The Smc5/6 complex is required for dissolution of DNA-mediated sister chromatid linkages 

      Bermúdez López, Marcelino; Ceschia, Audrey; Piccoli, Giacomo De; Colomina i Gabarrella, Neus; Pasero, Philippe; Aragón, Luis; Torres Rosell, Jordi (Oxford University Press, 2010)
      Mitotic chromosome segregation requires the removal of physical connections between sister chromatids. In addition to cohesin and topological entrapments, sister chromatid separation can be prevented by the presence of ...
    • Transcription of ribosomal genes can cause nondisjunction 

      Machín, Félix; Torres Rosell, Jordi; Piccoli, Giacomo De; Carballo, Jesús A.; Cha, Rita S.; Jarmuz, Adam; Aragón, Luis (The Rockefeller University Press, 2006)
      Mitotic disjunction of the repetitive ribosomal DNA (rDNA) involves specialized segregation mechanisms dependent on the conserved phosphatase Cdc14. The reason behind this requirement is unknown. We show that rDNA ...