The cell cycle–regulated genes of schizosaccharomyces pombe
MetadataShow full item record
Many genes are regulated as an innate part of the eukaryotic cell cycle, and a complex transcriptional network helps enable the cyclic behavior of dividing cells. This transcriptional network has been studied in Saccharomyces cerevisiae (budding yeast) and elsewhere. To provide more perspective on these regulatory mechanisms, we have used microarrays to measure gene expression through the cell cycle of Schizosaccharomyces pombe (fission yeast). The 750 genes with the most significant oscillations were identified and analyzed. There were two broad waves of cell cycle transcription, one in early/mid G2 phase, and the other near the G2/M transition. The early/mid G2 wave included many genes involved in ribosome biogenesis, possibly explaining the cell cycle oscillation in protein synthesis in S.pombe. The G2/M wave included at least three distinctly regulated clusters of genes: one large cluster including mitosis, mitotic exit, and cell separation functions, one small cluster dedicated to DNA replication, and another small cluster dedicated to cytokinesis and division. S. pombe cell cycle genes have relatively long, complex promoters containing groups of multiple DNA sequence motifs, often of two, three, or more different kinds. Many of the genes, transcription factors, and regulatory mechanisms are conserved between S. pombe and S. cerevisiae. Finally, we found preliminary evidence for a nearly genome-wide oscillation in gene expression: 2,000 or more genes undergo slight oscillations in expression as a function of the cell cycle, although whether this is adaptive, or incidental to other events in the cell, such as chromatin condensation, we do not know.
Is part ofPLoS Biology, 2005, vol. 3, núm. 7, p. 1239-1260
European research projects
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Ferrezuelo, Francisco; Aldea, Martí; Futcher, Bruce (Landes Bioscience, 2009)During the cell division cycle of the yeast Saccharomyces cerevisiae, the G1-to-S transition depends upon the activation of two transcription factors (SBF and MBF), which are responsible for the cell cycle-regulated ...
The transcriptional network activated by Cln3 cyclin at the G1-to-S transition of the yeast cell cycle Ferrezuelo, Francisco; Colomina i Gabarrella, Neus; Futcher, Bruce; Aldea, Martí (BioMed Central, 2010)Background: The G1-to-S transition of the cell cycle in the yeast Saccharomyces cerevisiae involves an extensive transcriptional program driven by transcription factors SBF (Swi4-Swi6) and MBF (Mbp1-Swi6). Activation of ...
Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin Pérez Fusté, Noel; Fernández Hernández, Rita; Cemeli, Tània; Mirantes Barbeito, Cristina; Pedraza González, Neus; Rafel i Borrell, Marta; Torres Rosell, Jordi; Colomina i Gabarrella, Neus; Ferrezuelo, Francisco; Dolcet Roca, Xavier; Garí Marsol, Eloi (Nature Publishing Group, 2016)Cyclin D1 (Ccnd1) together with its binding partner Cdk4 act as a transcriptional regulator to control cell proliferation and migration, and abnormal Ccnd1 Cdk4 expression promotes tumour growth and metastasis. While ...