Browsing Articles publicats (Medicina Experimental) by Issue Date
Now showing items 1-20 of 202
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Analysis of U1snRNP-specific A protein cross-linked complexes
(Wiley, 1990)The organization of the U1snRNP-specific A protein (34 kDa) has been analyzed by 12 and 16 A thiol-reversible chemical cross-linking and Western blotting. A-containing cross-linked complexes had molecular masses of 43, 47, ... -
Separation of rooster spermatogenic nuclei by means of centrifugal elutriation
(Wiley, 1993)By application of minor variations in the usual method of centrifugal elutriation, we have been able to separate a nuclear suspension from rooster testes. We have obtained a population of elongated or late spermatid ... -
Characterization of the Cell Death Process Induced by Staurosporine in Human Neuroblastoma Cell Lines
(Elsevier, 1997)Staurosporine is a potent and non-specific inhibitor of protein kinases. There is also evidence of staurosporine being a potent inducer of apoptosis. In several human neuroblastoma cell lines (SH-SY5Y, NB69, IMR-5 and ... -
Serum Deprivation and Protein Synthesis Inhibition Induce Two Different Apoptotic Processes in N18 Neuroblastoma Cells
(Elsevier, 1998)N18 are murine neuroblastoma cells that underwent cell death upon serum deprivation or inhibition of protein synthesis by means of cycloheximide (CHX). In both cases, an ultrastructural morphology and an internucleosomal ... -
The functional anatomy of the basal ganglia of birds
(Swets & Zeitlinger, 1999)To study how the basal ganglia can control movement in birds, we have reinvestigated the connections of the pigeon dorsal pallidum. Our results indicate that avian basal ganglia appear to control movement through major ... -
Neonatal tumor necrosis factor alpha promotes diabetes in nonobese diabetic mice by CD154-independent antigen presentation to CD8(+) T cells
(Rockefeller University Press, 2000)Neonatal islet-specific expression of tumor necrosis factor (TNF)-α in nonobese diabetic mice promotes diabetes by provoking islet-infiltrating antigen-presenting cells to present islet peptides to autoreactive T cells. ... -
Identification of the anterior nucleus of the ansa lenticularis in birds as the homologue of the mammalian subthalamic nucleus
(Society for Neuroscience, 2000)In mammals, the subthalamic nucleus (STN) is a glutamatergic diencephalic cell group that develops in the caudal hypothalamus and migrates to a position above the cerebral peduncle. By its input from the external pallidal ... -
Lipid rafts act as specialised domains for tetanus toxin binding and internalisation in neurons
(American Society for Cell Biology, 2001)Tetanus (TeNT) is a zinc protease that blocks neurotransmission by cleaving the synaptic protein vesicle-associated membrane protein/synaptobrevin. Although its intracellular catalytic activity is well established, the ... -
The prevention of the staurosporine-induced apoptosis by Bcl-XL, but not by Bcl-2 or caspase inhibitors, allows the extensive differentiation of human neuroblastoma cells
(Wiley, 2002)Staurosporine is one of the best apoptotic inducers in different cell types including neuroblastomas. In this study we have compared the efficiency and final outcome of three different anti-apoptotic strategies in ... -
Advanced glycation end product precursors impair epidermal growth factor receptor signalin
(American Diabetes Association, 2002-05)Formation of advanced glycation end products (AGEs) is considered a potential link between hyperglycemia and chronic diabetic complications, including disturbances in cell signaling. It was hypothesized that AGEs alter ... -
Functional analysis of the cysteine residues and the repetitive sequence of Saccharomyces cerevisiae Pir4/Cis3: the repetitive sequence is needed for binding to the cell wall beta-1,3-glucan.
(Wiley InterScience, 2003-08)Identification of PIR/CIS3 gene was carried out by amino-terminal sequencing of a protein band released by β-mercaptoethanol (β-ME) from S. cerevisiae mnn9 cell walls. The protein was released also by digestion with ... -
Loss of virulence in Ustilago maydis by Umchs6 gene disruption
(Elsevier, 2003-11-20)A gene encoding a sixth chitin synthase (Umchs6, sequence GenBank accession No. AF030554) from the plant pathogenic hemibasidiomycete Ustilago maydis (DC.) Cda. was isolated and characterized. The predicted protein is 1103 ... -
Cell differentiation, caspase inhibition and macromolecular synthesis blockage, but not Bcl-2 or Bcl-XL proteins, protect SH-SY5Y cells from apoptosis triggered by two cdk inhibitory drugs.
(Elsevier, 2004)Olomoucine and Roscovitine are two ATP-competing compounds described as specific inhibitors of cyclin-dependent kinases (CDK). Both drugs showed to induce apoptosis in SH-SY5Y, a neuroblastoma-derived cell line. In these ... -
The Avian Brain Nomenclature Forum: Terminology for a New Century in Comparative Neuroanatomy.
(Wiley, 2004)Many of the assumptions of homology on which the standard nomenclature for the cell groups and fiber tracts of avian brains have been based are in error, and as a result that terminology promotes misunderstanding of the ... -
Role of Pir1 in the construction of the Candida albicans cell wall
(Microbiology Society, 2004-10)Searches in a Candida albicans database (http://genolist.pasteur.fr/CandidaDB/) identified two Individual Protein Files (IPF 15363 and 19968) whose deduced amino acid sequences showed 42% and 45% homology with Saccharomyces ... -
Development of neurons and fibers containing calcium binding proteins in the pallial amygdala of mouse, with special emphasis on those of the basolateral amygdalar complex
(Wiley, 2005)We studied the development of neurons and fibers containing calbindin, calretinin, and parvalbumin in the mouse pallial amygdala, with special emphasis on those of the basolateral amygdalar complex. Numerous calbindin-im ... -
Embryonic and postnatal development of GABA, calbindin, calretinin, and parvalbumin in the mouse claustral complex
(Wiley, 2005)We analyzed the development of immunoreactive expression patterns for the neurotransmitter -aminobutyric acid (GABA) and the calcium-binding proteins calbindin, calretinin, and parvalbumin in the embryonic and postnatal ... -
Expression patterns of developmental regulatory genes show comparable divisions in the telencephalon of Xenopus and mouse: insights into the evolution of the forebrain
(Elsevier, 2005)In this study, we review data on the existence of comparable divisions and subdivisions in the telencephalon of different groups of tetrapods based on expression of some developmental regulatory genes, having a particular ... -
Distribution of nitric oxide-producing neurons in the developing and adult mouse amygdalar basolateral complex
(Elsevier, 2005)We analysed the expression of neuronal nitric oxide synthase (nNOS) in the mouse amygdalar basolateral complex (BLC) from embryonic day 15.5 to adult, using standard immunohistochemical methods. Our results indicate that ...