Show simple item record

dc.contributor.authorGómez-Pastor, Rocío
dc.contributor.authorPérez-Torrado, Roberto
dc.contributor.authorCabiscol Català, Elisa
dc.contributor.authorRos Salvador, Joaquim
dc.contributor.authorMatallana, Emilia
dc.date.accessioned2011-01-18T10:03:29Z
dc.date.available2011-01-18T10:03:29Z
dc.date.issued2010
dc.identifier.issn1475-2859
dc.identifier.urihttp://hdl.handle.net/10459.1/30297
dc.description.abstractBackground: Wine Saccharomyces cerevisiae strains, adapted to anaerobic must fermentations, suffer oxidative stress when they are grown under aerobic conditions for biomass propagation in the industrial process of active dry yeast production. Oxidative metabolism of sugars favors high biomass yields but also causes increased oxidation damage of cell components. The overexpression of the TRX2 gene, coding for a thioredoxin, enhances oxidative stress resistance in a wine yeast strain model. The thioredoxin and also the glutathione/glutaredoxin system constitute the most important defense against oxidation. Trx2p is also involved in the regulation of Yap1p-driven transcriptional response against some reactive oxygen species. Results: Laboratory scale simulations of the industrial active dry biomass production process demonstrate that TRX2 overexpression increases the wine yeast final biomass yield and also its fermentative capacity both after the batch and fed-batch phases. Microvinifications carried out with the modified strain show a fast start phenotype derived from its enhanced fermentative capacity and also increased content of beneficial aroma compounds. The modified strain displays an increased transcriptional response of Yap1p regulated genes and other oxidative stress related genes. Activities of antioxidant enzymes like Sod1p, Sod2p and catalase are also enhanced. Consequently, diminished oxidation of lipids and proteins is observed in the modified strain, which can explain the improved performance of the thioredoxin overexpressing strain. Conclusions: We report several beneficial effects of overexpressing the thioredoxin gene TRX2 in a wine yeast strain. We show that this strain presents an enhanced redox defense. Increased yield of biomass production process in TRX2 overexpressing strain can be of special interest for several industrial applications.ca_ES
dc.language.isoengca_ES
dc.publisherBioMed Centralca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1186/1475-2859-9-9
dc.relation.ispartofMicrobial Cell Factories, 2010, vol. 9, núm. 9, p. 1-14ca_ES
dc.rightscc-by, (c) Gómez-Pastor et al., 2010ca_ES
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/es/deed.caca_ES
dc.subject.otherVi -- Llevatsca_ES
dc.subject.otherVi -- Oxidacióca_ES
dc.titleReduction of oxidative cellular damage by overexpression of the thioredoxin TRX2 gene improves yield and quality of wine yeast dry active biomassca_ES
dc.typearticleca_ES
dc.identifier.idgrec015121
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1186/1475-2859-9-9


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

cc-by, (c) Gómez-Pastor et al., 2010
Except where otherwise noted, this item's license is described as cc-by, (c) Gómez-Pastor et al., 2010