Now showing items 1-7 of 7

    • An in vitro system for the rapid functional characterization of genes involved in carotenoid biosynthesis and accumulation 

      Bai, Chao; Rivera Vélez, Sol Maiam; Medina Piles, Vicente; Alves, Rui; Vilaprinyo Terré, Ester; Sorribas Tello, Albert; Canela i Garayoa, Ramon; Capell Capell, Teresa; Sandmann, Gerhard; Christou, Paul; Zhu, Changfu (Wiley, 2014)
      We have developed an assay based on rice embryogenic callus for rapid functional characterization of metabolic genes. We validated the assay using a selection of well-characterized genes with known functions in the carotenoid ...
    • Analytical tools for the analysis of carotenoids in diverse materials 

      Rivera Vélez, Sol Maiam; Canela i Garayoa, Ramon (Elsevier, 2012)
      High-performance liquid chromatography (HPLC) has become the method of choice for carotenoid analysis. Although a number of normal-phase columns have been reported, reverse-phase columns are the most widely used stationary ...
    • Determination of carotenoids by liquid chromatography/mass spectrometry: effect of several dopants 

      Rivera Vélez, Sol Maiam; Vilaró, Francisca; Canela i Garayoa, Ramon (Springer Verlag, 2011)
      Various carotenoids were analyzed by ultra-high-pressure liquid chromatography with tandem mass spectrometry detection (UHPLC-MS/MS). Three different techniques to ionize the carotenoids were compared: electrospray ionization ...
    • Fast quantitative method for the analysis of carotenoids in transgenic maize 

      Rivera Vélez, Sol Maiam; Vilaró, Francisca; Zhu, Changfu; Bai, Chao; Farré Martinez, Gemma; Christou, Paul; Canela i Garayoa, Ramon (American Chemical Society, 2013)
      A fast method was developed to determine carotenoid content in transgenic maize seeds. The analysis was carried out using an ultrahigh-pressure liquid chromatograph coupled to a photodiode array detector and a mass ...
    • Identification of carotenoids using mass spectrometry 

      Rivera Vélez, Sol Maiam; Christou, Paul; Canela i Garayoa, Ramon (Wiley Periodicals, 2014)
      The present review compiles positive MS fragmentation data of selected carotenoids obtained using various ionization techniques and matrices. In addition, new experimental data from the analysis of carotenoids in transgenic ...
    • Influence of sample processing on the analysis of carotenoids in maize 

      Rivera Vélez, Sol Maiam; Canela i Garayoa, Ramon (Molecular Diversity Preservation International, 2012)
      We performed a number of tests with the aim to develop an effective extraction method for the analysis of carotenoid content in maize seed. Mixtures of methanol–ethyl acetate (6:4, v/v) and methanol–tetrahydrofuran (1:1, ...
    • Targeted transcriptomic and metabolic profiling reveals temporal bottlenecks in the maize carotenoid pathway that may be addressed by multigene engineering 

      Farré Martinez, Gemma; Rivera Vélez, Sol Maiam; Alves, Rui; Vilaprinyo Terré, Ester; Sorribas Tello, Albert; Canela i Garayoa, Ramon; Naqvi, Shaista; Sandmann, Gerhard; Capell Capell, Teresa; Zhu, Changfu; Christou, Paul (Wiley, 2013)
      Carotenoids are a diverse group of tetraterpenoid pigments found in plants, fungi, bacteria and some animals. They play vital roles in plants and provide important health benefits to mammals, including humans. We ...