Browsing Articles publicats (Ciència Animal) by Author "de la Fuente Oliver, Gabriel"
Now showing items 1-20 of 38
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A cryopreservation procedure for the rumen protozoon Entodinium caudatum: estimation of its viability by fluorescence microscopy
de la Fuente Oliver, Gabriel; Cebrián, J. A.; Fondevila, Manuel (Blackwell Science, 2004)Aims: To study the viability of a culture of the rumen protozoon Entodinium caudatum after a cryopreservation procedure by a fluorescence microscopy staining method. Methods and Results: Fluorescence method is based on ... -
Analysis of stomach bacterial communities in Australian feral horses
Benoit, St-Pierre; de la Fuente Oliver, Gabriel; Sean, O'Neill; André-Denis, G. Wright; Rafat, Al Jassim (Springer Verlag, 2012-10-13)We investigated the community structure of bacteria that populate the stomach of the Brumby, a breed of feral horses from the Australian outback. Using a 16S rRNA gene clone library, we identified 155 clones that were ... -
Antiprotozoal effect of saponins in the rumen can be enhanced by chemical modifications in their structure
Ramos-Morales, Eva; de la Fuente Oliver, Gabriel; Duval, Stéphane; Wehrli, Christof; Bouillon, Marc E.; Lahmann, Martina; Preskett, David; Braganca, Radek; Newbold, C. Jamie (Frontiers Media, 2017-02-27)The antiprotozoal effect of saponins is transitory, as when saponins are deglycosylated to the sapogenin by rumen microorganisms they become inactive. We postulated that the substitution of the sugar moiety of the saponin ... -
Bacterial protein degradation by different rumen protozoal groups
Belanche, A.; de la Fuente Oliver, Gabriel; Moorby, J. M.; Newbold, C. Jamie (American Society of Animal Science, 2012-07-08)Bacterial predation by protozoa has the most deleterious effect on the efficiency of N use within the rumen, but differences in activity among protozoal groups are not completely understood. Two in vitro experiments were ... -
Biological effect of tannins from different vegetal origin on microbial and fermentation traits in vitro
Rodríguez Ochoa, Rafael; de la Fuente Oliver, Gabriel; Gómez, S.; Fondevila, Manuel (CSIRO Publishing, 2013-11-26)The biological effect of tannins (proportion of the response in different parameters when tannins were inactivated with polyethylene glycol, PEG) as an easy, rapid way to estimate the magnitude of their effect on rumen ... -
Characterisation of the faecal bacterial community in adult and elderly horses fed a high fibre, high oil or high starch diet using 454 pyrosequencing
Dougal, Kirsty; de la Fuente Oliver, Gabriel; Harris, Patricia A.; Girdwood, Susan E.; Pinloche, Eric; Raymond J. Geor; Nielsen, Brian D.; Schott II, Harold C.; Elzinga, Sarah; Newbold, C. Jamie (Public Library of Science, 2014-02-04)Faecal samples were collected from seventeen animals, each fed three different diets (high fibre, high fibre with a starch rich supplement and high fibre with an oil rich supplement). DNA was extracted and the V1-V2 regions ... -
Ciliate protozoa of the forestomach of llamas (Lama glama) from locations at different altitude in Argentina
Cerón Cucchi, María; Marcoppido, Gisela; Dekker, Anna; Fondevila, Manuel; de la Fuente Oliver, Gabriel; Morici, Gabriel; Cravero, Silvio (Magnolia Press, 2016-01-20)This study describes the diversity and concentration of the protozoal population from the forestomach of llamas in Argentina at three altitudinal locations. Protozoal diversity was studied in samples from eight llamas from ... -
Clinical and Echocardiographic Findings in an Aged Population of Cavalier King Charles Spaniels
Prieto Ramos, Jorge; Corda, Andrea; Swift, Simon; Saderi, Laura; de la Fuente Oliver, Gabriel; Corcoran, Brendan; Summers, Kim M.; French, Anne T. (MDPI, 2021-03-28)Myxomatous mitral valve disease (MMVD) is the most common cardiac disease in dogs. It varies from dogs without clinical signs to those developing left-sided congestive heart failure, leading to death. Cavalier King Charles ... -
Description of development of rumen ecosystem by PCR assay in milk-fed, weaned and finished lambs in an intensive fattening system
Belanche, A.; Balcells Terés, Joaquim; de la Fuente Oliver, Gabriel; Yáñez-Ruiz, D. R.; Fondevila, Manuel; Calleja, L. (John Wiley & SonsWilley Blackwell, 2010-09-14)This study examined the reticulo-rumen characteristics of the microbial community and its fermentative characteristics in milk-fed, at weaning and finished lambs in a conventional fattening system. Five lambs were assigned ... -
Effect of diet and absence of protozoa on the rumen microbial community and on the representativeness of bacterial fractions used in the determination of microbial protein synthesis
Belanche, A.; de la Fuente Oliver, Gabriel; Pinloche, Eric; Newbold, C. Jamie; Balcells Terés, Joaquim (American Society of Animal Science, 2012-04-21)Accurate estimates of microbial synthesis in the rumen are vital to optimize ruminant nutrition. Liquid- (LAB) and solid-associated bacterial fractions (SAB) harvested from the rumen are generally considered as microbial ... -
Effect of progressive inoculation of fauna-free sheep with holotrich protozoa and total-fauna on rumen fermentation, microbial diversity and methane emissions
Belanche, A.; de la Fuente Oliver, Gabriel; Newbold, C. Jamie (Oxford University Press, 2014-12-15)Rumen methanogenesis represents an energy waste for the ruminant and an important source of greenhouse gas; thus, integrated studies are needed to fully understand this process. Eight fauna-free sheep were used to investigate ... -
Effect of the cryopreservation conditions on the viability of the rumen ciliate Diploplastron (Metadinium) affine
de la Fuente Oliver, Gabriel; Cebrián, J. A.; Fondevila, Manuel (Blackwell Science, 2006)Aims: To study the viability of Diploplastron (Metadinium) affine after its cryopreservation at two cooling rates, and the effect of procedure conditions on viability. Methods and Results: There were differences in ... -
Effect of type roughage and level of barley supplementation on digestibility, rumen fermentation and microbial-N yield
Belanche, A.; de la Fuente Oliver, Gabriel; Guada, J. A.; Balcells Terés, Joaquim (Centre international de hautes études agronomiques méditerranéennes, 2011)Four cannulated ewes were used in a factorial (2 x 3) design to study the effect of two sources of roughage (alfalfa hay vs ammonium-treated straw, UTS) and three levels of ground barley grain supplementation (F:C ratio: ... -
Effects of protein restriction on performance, ruminal fermentation and microbial community in Holstein bulls fed high-concentrate diets
Costa-Roura, Sandra; Balcells Terés, Joaquim; de la Fuente Oliver, Gabriel; Mora-Gil, Jesús; Llanes, Núria; Villalba Mata, Daniel (Elsevier, 2020-03-24)The aim of this work was to study the impact of a crude protein (CP) restriction on performance, ruminal fermentation and microbial community composition in fattening Holstein bulls (from 118 to 331 d of age and from 165 ... -
Estudio in vitro de la fermentación ruminal de carbohidratos en cabra montés
de la Fuente Oliver, Gabriel; Belanche, A.; Fondevila, Manuel (Universidad de Córdoba. Asociación Iberoamericana de Zootecnia, 2009-09-01)The 48 h fermentation pattern of pectin, xylan and cellulose from rumen contents of males (MM), females (MH) and kids (MJ) of Spanish Ibex (SI) and from domestic goats (CD) was studied. There were no differences (p>0.05) ... -
Functional resilience and response to a dietary additive (Kefir) in models of foregut and hindgut microbial fermentation in vitro
de la Fuente Oliver, Gabriel; Jones, Eleanor; Jones, Shann; Newbold, C. Jamie (Frontiers Media S.A., 2017-06-28)Stability in gut ecosystems is an important area of study that impacts on the use of additives and is related with several pathologies. Kefir is a fermented milk drink made with a consortium of yeast and bacteria as a ... -
Identification of a core bacterial community within the large intestine of the horse
Dougal, Kirsty; de la Fuente Oliver, Gabriel; Harris, Patricia A.; Girdwood, Susan E.; Pinloche, Eric; Newbold, C. Jamie (Public Library of Science, 2013-10-24)The horse has a rich and complex microbial community within its gastrointestinal tract that plays a central role in both health and disease. The horse receives much of its dietary energy through microbial hydrolysis and ... -
Improving the antiprotozoal effect of saponins in the rumen by combination with glycosidase inhibiting iminosugars or by modification of their chemical structure
Ramos-Morales, Eva; de la Fuente Oliver, Gabriel; Nash, Robert J.; Braganca, Radek; Duval, Stéphane; Bouillon, Marc E.; Lahmann, Martina; Newbold, C. Jamie (Public Library of Science, 2017)The antiprotozoal effect of saponins is transitory, as when saponins are deglycosylated to sapogenins by rumen microorganisms they become inactive. We hypothesised that the combination of saponins with glycosidase-inhibiting ... -
In vitro and in situ degradation characteristics and rumen fermentation products of moringa oleifera harvested at three different ages
Seradj, Ahmad Reza; Morazán Nuñez, Henris Jobany; Fondevila, Manuel; Liang, J. B.; de la Fuente Oliver, Gabriel; Balcells Terés, Joaquim (Bogor Agricultural University, 2019-04)Besides the beneficial agronomic properties such as drought tolerance and high biomass production, the high crude protein content of Moringa oleifera (Moringa) makes it comparable to the other high quality forages. This ... -
In vitro predation of pure bacterial species by rumen protozoa from monofaunated sheep, determined by qPCR
de la Fuente Oliver, Gabriel; Morgavi, Diego P.; Belanche, A.; Fondevila, Manuel (Centre international de hautes études agronomiques méditerranéennes, 2011)Predation of bacteria has important implications on rumen microbial ecology and metabolism. The predation rate of bacteria by protozoa in vitro was studied by bacterial DNA quantification. Entodinium caudatum (EC), Diplodinium ...