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    • Validation of sheep rumen fluid frozen in liquid N as inoculum for in vitro gas production trials. 

      Prates, A.; de Oliveira, J.A.; de la Fuente Oliver, Gabriel; Abecia, Leticia; Fondevila, Manuel (Centre international de hautes études agronomiques méditerranéennes, 2011)
      The effect of freezing rumen fluid in liquid nitrogen for its use as inoculum for in vitro incubation was evaluated in a semi-continuous system. Inoculum was used immediately (RF) or frozen and then thawed and used (TW) ...