Browsing Articles publicats (Ciència Animal) by Author "Newbold, C. Jamie"
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Antiprotozoal effect of saponins in the rumen can be enhanced by chemical modifications in their structure
Ramos-Morales, Eva; de la Fuente Oliver, Gabriel; Duval, Stéphane; Wehrli, Christof; Bouillon, Marc E.; Lahmann, Martina; Preskett, David; Braganca, Radek; Newbold, C. Jamie (Frontiers Media, 2017-02-27)The antiprotozoal effect of saponins is transitory, as when saponins are deglycosylated to the sapogenin by rumen microorganisms they become inactive. We postulated that the substitution of the sugar moiety of the saponin ... -
Bacterial protein degradation by different rumen protozoal groups
Belanche, A.; de la Fuente Oliver, Gabriel; Moorby, J. M.; Newbold, C. Jamie (American Society of Animal Science, 2012-07-08)Bacterial predation by protozoa has the most deleterious effect on the efficiency of N use within the rumen, but differences in activity among protozoal groups are not completely understood. Two in vitro experiments were ... -
Characterisation of the faecal bacterial community in adult and elderly horses fed a high fibre, high oil or high starch diet using 454 pyrosequencing
Dougal, Kirsty; de la Fuente Oliver, Gabriel; Harris, Patricia A.; Girdwood, Susan E.; Pinloche, Eric; Raymond J. Geor; Nielsen, Brian D.; Schott II, Harold C.; Elzinga, Sarah; Newbold, C. Jamie (Public Library of Science, 2014-02-04)Faecal samples were collected from seventeen animals, each fed three different diets (high fibre, high fibre with a starch rich supplement and high fibre with an oil rich supplement). DNA was extracted and the V1-V2 regions ... -
Effect of diet and absence of protozoa on the rumen microbial community and on the representativeness of bacterial fractions used in the determination of microbial protein synthesis
Belanche, A.; de la Fuente Oliver, Gabriel; Pinloche, Eric; Newbold, C. Jamie; Balcells Terés, Joaquim (American Society of Animal Science, 2012-04-21)Accurate estimates of microbial synthesis in the rumen are vital to optimize ruminant nutrition. Liquid- (LAB) and solid-associated bacterial fractions (SAB) harvested from the rumen are generally considered as microbial ... -
Effect of progressive inoculation of fauna-free sheep with holotrich protozoa and total-fauna on rumen fermentation, microbial diversity and methane emissions
Belanche, A.; de la Fuente Oliver, Gabriel; Newbold, C. Jamie (Oxford University Press, 2014-12-15)Rumen methanogenesis represents an energy waste for the ruminant and an important source of greenhouse gas; thus, integrated studies are needed to fully understand this process. Eight fauna-free sheep were used to investigate ... -
Functional resilience and response to a dietary additive (Kefir) in models of foregut and hindgut microbial fermentation in vitro
de la Fuente Oliver, Gabriel; Jones, Eleanor; Jones, Shann; Newbold, C. Jamie (Frontiers Media S.A., 2017-06-28)Stability in gut ecosystems is an important area of study that impacts on the use of additives and is related with several pathologies. Kefir is a fermented milk drink made with a consortium of yeast and bacteria as a ... -
Identification of a core bacterial community within the large intestine of the horse
Dougal, Kirsty; de la Fuente Oliver, Gabriel; Harris, Patricia A.; Girdwood, Susan E.; Pinloche, Eric; Newbold, C. Jamie (Public Library of Science, 2013-10-24)The horse has a rich and complex microbial community within its gastrointestinal tract that plays a central role in both health and disease. The horse receives much of its dietary energy through microbial hydrolysis and ... -
Improving the antiprotozoal effect of saponins in the rumen by combination with glycosidase inhibiting iminosugars or by modification of their chemical structure
Ramos-Morales, Eva; de la Fuente Oliver, Gabriel; Nash, Robert J.; Braganca, Radek; Duval, Stéphane; Bouillon, Marc E.; Lahmann, Martina; Newbold, C. Jamie (Public Library of Science, 2017)The antiprotozoal effect of saponins is transitory, as when saponins are deglycosylated to sapogenins by rumen microorganisms they become inactive. We hypothesised that the combination of saponins with glycosidase-inhibiting ... -
Not all saponins have a greater antiprotozoal activity than their related sapogenins
Ramos-Morales, Eva; Lyons, Laura; de la Fuente Oliver, Gabriel; Braganca, Radek; Newbold, C. Jamie (Oxford University Press, 2019-07-04)The antiprotozoal effect of saponins varies according to both the structure of the sapogenin and the composition and linkage of the sugar moieties to the sapogenin. The effect of saponins on protozoa has been considered ... -
Pros and cons of ion-torrent next generation sequencing versus terminal restriction fragment length polymorphism T-RFLP for studying the rumen bacterial community
de la Fuente Oliver, Gabriel; Belanche, A.; Girwood, Susan E.; Pinloche, Eric; Wilkinson, Toby; Newbold, C. Jamie (Public Library of Science, 2014-07-22)The development of next generation sequencing has challenged the use of other molecular fingerprinting methods used to study microbial diversity. We analysed the bacterial diversity in the rumen of defaunated sheep following ... -
Study of methanogen communities associated with different rumen protozoal populations
Belanche, A.; de la Fuente Oliver, Gabriel; Newbold, C. Jamie (Oxford University Press, 2015-01-08)Protozoa-associated methanogens (PAM) are considered one of the most active communities in the rumen methanogenesis. This experiment investigated whether methanogens are sequestrated within rumen protozoa, and structural ... -
Technical note: The persistence of microbial-specific DNA sequences through gastric digestion in lambs and their potential use as microbial markers
Belanche, A.; de la Fuente Oliver, Gabriel; Yáñez-Ruiz, D. R.; Newbold, C. Jamie; Calleja, L.; Balcells Terés, Joaquim (American Society of Animal Science, 2011-04-12)Two groups of 5 lambs were euthanized at the weaning (T45) and fattening stages (T90) to evaluate the use of microbial ribosomal DNA (rDNA) sequences as potential microbial markers in relation to purine bases (PB) as a ... -
The physical form of corn influences the rumen bacterial biodiversity-preliminary results
Rossi, G.; Tagliapietra, F.; Pinloche, Eric; de la Fuente Oliver, Gabriel; Schiavon, S.; Maccarana, L.; Bittante, G.; Newbold, C. Jamie (Faculty of Agriculture in Osijek, Agricultural Institute Osijek, 2015-06)The aim of this study was to investigate the rumen bacteria in terms of genetic biodiversity and variation due to different physical form of corn in cow diet. A total of twenty dry cows were fed for 3 months with the same ... -
The role of ciliate protozoa in the rumen
Newbold, C. Jamie; de la Fuente Oliver, Gabriel; Belanche, A.; Ramos-Morales, Eva; McEwan, Neil (Frontiers, 2015-11-09)First described in 1843, Rumen protozoa with their striking appearance were assumed to be important for the welfare of their host. However, despite contributing up to 50% of the bio-mass in the rumen, the role of protozoa ...