El Centre de Ciència i Tecnologia Forestal de Catalunya (CTFC), amb seu a Solsona (Lleida), es va constituir inicialment entre el Consell Comarcal del Solsonès i la Universitat de Lleida l'any 1996, i posteriorment, s'hi van incorporar la Diputació de Lleida, la Fundació Catalana per a la Recerca i la Innovació, la Generalitat de Catalunya, la Universitat Autònoma de Barcelona, la Diputació de Barcelona i l'Ajuntament de Solsona.
El CTFC és un consorci públic adscrit a l’Administració de la Generalitat de Catalunya i s’hi relaciona mitjançant el Departament competent en matèria de boscos. També forma part dels centres de recerca de Catalunya (CERCA) i està acreditat com agent TECNIO per la Generalitat (desenvolupador de tecnologia públic). [Més informació]
Browsing Centre de Ciència i Tecnologia Forestal de Catalunya (CTFC) by Subject "16S rRNA"
Anthropogenic disturbance has the potential to negatively afect wildlife health by altering food availability and diet composition, increasing the exposure to agrochemicals, and intensifying the contact with humans, domestic animals, and their pathogens. However, the impact of these factors on the fecal microbiome composition of wildlife hosts and its link to host health modulation remains barely explored. Here we investigated the composition of the fecal bacterial microbiome of the insectivorous bat Kuhl’s pipistrelle (Pipistrellus kuhlii) dwelling in four environmental contexts with diferent levels of anthropogenic pressure. We analyzed their microbiome composition, structure and diversity through full-length 16S rRNA metabarcoding using the nanopore long-read sequencer MinION™. We hypothesized that the bacterial community structure of fecal samples would vary across the diferent scenarios, showing a decreased diversity and richness in samples from disturbed ecosystems.
The fecal microbiomes of 31 bats from 4 scenarios were sequenced. A total of 4,829,302 reads were obtained with a taxonomic assignment percentage of 99.9% at genus level. Most abundant genera across all scenarios were Enterococcus, Escherichia/Shigella, Bacillus and Enterobacter. Alpha diversity varied signifcantly between the four scenarios (p<0.05), showing the lowest Shannon index in bats from urban and intensive agriculture landscapes, while the highest alpha diversity value was found in near pristine landscapes. Beta diversity obtained by Bray–Curtis distance showed weak statistical diferentiation of bacterial taxonomic profles among scenarios. Furthermore, core community analysis showed that 1,293 genera were shared among localities. Diferential abundance analyses showed that the highest diferentially abundant taxa were found in near pristine landscapes, with the exception of the family
Alcaligenaceae, which was also overrepresented in urban and intensive agriculture landscapes.
This study suggests that near pristine and undisturbed landscapes could promote a more resilient gutmicrobiome in wild populations of P. kuhlii. These results highlight the potential of the fecal microbiome as a noninvasive bioindicator to assess insectivorous bats’ health and as a key element of landscape conservation strategies.