Esterification of oleic acid catalayzed by an Aspergillus niger strain. Influence of water activity and alcohol concentration
Méndez, Jonh Jairo
Oromí Farrús, Mireia
Loscos i Solé, Vicent
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Effects of water activity and 1-propanol concentration on synthesis of propyl oleate from oleic acid using Aspergillus niger cell-bound lipases in isooctane are described. A. niger produces lipases (EC 18.104.22.168) which partly bind to the mycelium during growth. Ester production was monitored for 72 hours at different 1-propanol concentrations and water activities. Aliquots were sequentially withdrawn and propyl esters were quantified using GC and methyl palmitate as an internal standard. In all assayed conditions A. niger cell-bound lipases catalysed propyl oleate synthesis, but at different degrees.